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Genetics of the peroxidase isoenzymes in Petunia : Part 1: organ specificity and general genetic aspects of the peroxidase isoenzymes.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
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Genetics of the peroxidase isoenzymes in Petunia : Part 2: Location and developmental expression of the structural gene prxB.

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Genetics of the peroxidase isoenzymes in Petunia : Part 5. Differential temporal expression of prxA alleles.

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Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
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Evidence for transposition in Petunia.

H J Wijsman1

  • 1Institute of Genetics, University of Amsterdam, Kruislaan 318, NL-1098 SM, Amsterdam, The Netherlands.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|November 20, 2013
PubMed
Summary

Genetic element Bi interacts with responsive anthocyanin alleles in Petunia, creating variegated flower colors. Loss of Bi results in white flowers, revealing responsive alleles and a weak Bi mutant.

Area of Science:

  • Plant genetics
  • Molecular biology
  • Flower coloration studies

Background:

  • Flower color in Petunia is influenced by genetic interactions.
  • Trans-regulatory elements play a role in controlling gene expression.
  • Anthocyanin pigments are key determinants of flower pigmentation.

Purpose of the Study:

  • To investigate the interaction between the trans-regulatory genetic element Bi and responsive anthocyanin alleles in Petunia.
  • To characterize the genetic basis of variegated flower color patterns.
  • To identify and describe a weak mutant of the Bi element.

Main Methods:

  • Genetic crosses in Petunia to analyze segregation patterns.
  • Phenotypic analysis of flower color in different genetic lines.

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  • Mapping of the Bi element to specific chromosomes.
  • Main Results:

    • Demonstrated interaction between the Bi element and responsive anthocyanin alleles leads to variegated flower color.
    • Identified white-flowering segregants lacking the Bi element, confirming the presence of responsive alleles.
    • Described a weak mutant of the Bi element with altered function.
    • Determined that the Bi element is present in some Petunia lines and absent in others.
    • Mapped the Bi element to chromosome I in two specific Petunia lines.

    Conclusions:

    • The Bi element is a trans-regulatory factor controlling anthocyanin expression in Petunia.
    • Varied flower color patterns result from the interplay between Bi and specific anthocyanin alleles.
    • The Bi element's presence and function can be genetically mapped and mutated.