Variability of DNA content and karyotype in cell cultures of an interdihaploid Solanum tuberosum
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Updated: May 5, 2026
Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts
Published on: March 9, 2018
L P Pijnacker1, J H Hermelink, M A Ferwerda
1Department of Genetics, University of Groningen, Centre of Biological Sciences, P.O. Box 14, NL-9750 AA, Haren, The Netherlands.
Abstract:
Karyological and DNA cytophotometric observations were carried out to follow nuclear events in cell cultures of an interdihaploid potato, genotype H(2)578, 2n=2x=24. The percentage of polyploid cells increased in leaf calluses, but decreased and then remained at a constant level when these cells were cultured in suspension. These suspension cultures did not survive, maybe because they did not become tetraploid. Another suspension culture proliferated with hypotetraploid cells only. Unlike the DNA contents of their interphase nuclei, the karyotypes varied considerably because of numerical and structural chromosome mutations.
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