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Plant regeneration from shoot callus of rosewood (Dalbergia latifolia Roxb.)
G Lakshmi Sita1, S Chattopadhyay, D H Tejavathi
1Microbiology and Cell Biology Laboratory, Indian Institute of Science, 560 012, Bangalore, India.
Abstract:
In vitro propagation of trees using cell, tissue and organ culture is a fast emerging area. We report here the clonal propagation of Indian rosewood (Dalbergia latifolia Roxb.) from shoot callus cultures of 5 year old trees. Bud regeneration was obtained on MS media supplemented with BA and NAA. About 35% of the cultures showed organogenesis. Shoots measuring about 3-5 cm can be excised and rooted in White's medium supplemented with 1-2 mg/L IAA. Rooted plants were successfully established in soil.
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