Assay of membrane-associated antigens with monoclonal antibody

Beta-galactosidase conjugated to erythrocyte vesicles was measured by enzymatic and immunologic methods. The immunoassay used a monoclonal antibody and an anti-mouse IgG antibody conjugated to alkaline phosphatase in an adaptation of the enzyme-linked immunosorbent assay. The beta-galactosidase and alkaline phosphatase activities were compared by correlation. The significant correlation indicates that this method is satisfactory for relative quantitation of membrane-associated antigens with exposed epitopes.