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Medicago cell variants showing altered nitrogen utilization.

G Giuliano1, G Mita, S E Indiogine

  • 1Istituto di Mutagenesi e Differenziamento, CNR, Via Svezia 10, I-56100, Pisa, Italy.

Plant Cell Reports
|November 20, 2013
PubMed
Summary
This summary is machine-generated.

Mutagenesis of Medicago coerulea identified chlorate-resistant variants with altered nitrate reductase activity. Methylammonium-resistant variants displayed distinct nitrogen utilization regulation, showing less ammonium repression of nitrate reductase.

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Area of Science:

  • Plant molecular biology
  • Nitrogen metabolism in plants
  • Plant genetics

Background:

  • Nitrate reductase is a key enzyme in plant nitrogen assimilation.
  • Ammonium is known to repress nitrate reductase activity in wild-type plant cells.
  • Understanding nitrogen utilization pathways is crucial for plant physiology and agriculture.

Purpose of the Study:

  • To isolate and characterize chlorate-resistant and methylammonium-resistant variants of Medicago coerulea.
  • To investigate the regulation of nitrogen utilization pathways in these variants.
  • To identify genetic or regulatory changes affecting nitrate reductase and other nitrogen metabolism enzymes.

Main Methods:

  • Mutagenesis of Medicago coerulea cells.
  • Isolation of chlorate-resistant and methylammonium-resistant variants.
  • Assay of nitrate reductase activity.
  • Analysis of xanthine dehydrogenase activity and adenine uptake.

Main Results:

  • Nineteen chlorate-resistant variants showed variable nitrate reductase activity (5-100% of wild type); xanthine dehydrogenase was unaffected.
  • Methylammonium-resistant variants exhibited altered regulation of nitrogen utilization.
  • These variants displayed reduced sensitivity to ammonium repression of nitrate reductase activity compared to wild-type cells.

Conclusions:

  • Mutagenesis can generate variants with altered nitrogen assimilation pathways in Medicago coerulea.
  • The identified variants provide insights into the complex regulation of nitrate reductase and nitrogen utilization.
  • These findings contribute to understanding plant responses to nitrogen availability and potential applications in crop improvement.