Related Experiment Video
Updated: May 5, 2026

Author Spotlight: A Simple and Cost-Effective Method for Leukocyte Analysis in Semen
Published on: January 19, 2024
Some characteristics of peroxidase secreted by cotton ovule cultures
1US Department of Agriculture, Agriculture Research Service, Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA, USA.
Abstract:
Cotton (Gossypium hirsutum L.) ovule cultures secreted a soluble peroxidase into the surrounding medium, resulting in a 200-fold increase in this activity during the 30-day growth period. The peroxidase activity was thermostable from 4°C to 60°C and displayed a pH optimum of 5.5 to 6.0. The ovule peroxidase was susceptible to periodate treatment and very resistant to protease digestion. The data suggest that the peroxidase activity is a glycoprotein. Interpretation of peroxidase data may be complicated by the presence of phenol oxidase activity in the same preparations. The presence of phenol oxidases was ruled out by the inaction of a tyrosinase-specific inhibitor, tropolone.
Related Concept Videos
Peroxisomes
Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
Autoxidation of Ethers to Peroxides and Hydroperoxides

