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High-Throughput, Multi-Image Cryohistology of Mineralized Tissues
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Long term storage of callus cultures at low temperatures or under mineral oil layer
J M Augereau1, D Courtois, V Petiard
1L.E.R. Synthelabo, BP 0166, F-37001, Tours Cedex, France.
Plant Cell Reports
|November 20, 2013
Abstract:
Plant tissue cultures from various species were stored at low temperatures or under mineral oil overlay for 4 to 6 months without subcultures. After transfer to normal culture conditions, it was checked, with 3 strains, that growth characteristics and secondary metabolite production were preserved. The storage with a mineral oil overlay (easy to run and economical method) could be a possible alternative to cryogenic or low temperature storage for a large number of strains.

