Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.4K
Next-generation Sequencing03:00

Next-generation Sequencing

88.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.0K
Sanger Sequencing01:57

Sanger Sequencing

801.1K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
801.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A receptor-like mechanosensitive protein governs preprophase band positioning for asymmetric cell divisions and SC morphogenesis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Nuclear condensates of BZU2/ZmMUTE modulate transcription to realize structural heterogeneity of the maize stomatal complex.

The Plant cell·2026
Same author

Exploring the maize transcriptional regulatory landscape through large-scale profiling of transcription factor binding sites.

Molecular plant·2025
Same author

Decoding the gene regulatory network of endosperm differentiation in maize.

Nature communications·2024
Same author

A maize epimerase modulates cell wall synthesis and glycosylation during stomatal morphogenesis.

Nature communications·2023
Same author

From mouse to mouse-ear cress: Nanomaterials as vehicles in plant biotechnology.

Exploration (Beijing, China)·2023
Same journal

The TaMYB55-TaSnRK1α1-TabZIP9 module confers heat stress tolerance in wheat.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Superstatistics approach to turbulent circulation fluctuations.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

A molecular timescale for evolution of cobamide biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Pierre Chambon, a pioneer of molecular biology and gene regulation in eukaryotes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Granulosa cell glycogen fuels the avascular corpus luteum.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Synthetic essentiality of TRAIL/TNFSF10 in VHL-deficient renal cell carcinoma.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: May 5, 2026

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
07:12

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Published on: July 28, 2023

4.8K

RNA-sequencing from single nuclei.

Rashel V Grindberg1, Joyclyn L Yee-Greenbaum, Michael J McConnell

  • 1J. Craig Venter Institute, San Diego, CA 92121.

Proceedings of the National Academy of Sciences of the United States of America
|November 20, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a new transcriptomic analysis method using single nuclei, enabling gene expression quantification from minimal mRNA. This technique offers high coverage and reproducibility, advancing single-cell level gene regulation studies.

Keywords:
deep sequencingnuclear RNAwhole cell RNA

More Related Videos

Author Spotlight: Deciphering the Cellular Mysteries of Intermuscular Adipose Tissue in Humans
05:59

Author Spotlight: Deciphering the Cellular Mysteries of Intermuscular Adipose Tissue in Humans

Published on: May 3, 2024

1.3K
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

12.3K

Related Experiment Videos

Last Updated: May 5, 2026

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
07:12

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Published on: July 28, 2023

4.8K
Author Spotlight: Deciphering the Cellular Mysteries of Intermuscular Adipose Tissue in Humans
05:59

Author Spotlight: Deciphering the Cellular Mysteries of Intermuscular Adipose Tissue in Humans

Published on: May 3, 2024

1.3K
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

12.3K

Area of Science:

  • Molecular Biology
  • Genomics
  • Neuroscience

Background:

  • Single-cell RNA sequencing (scRNA-seq) allows global gene expression quantification.
  • Analyzing gene expression from single cells reveals cellular heterogeneity.
  • Challenges remain in isolating sufficient RNA from certain cell types or tissues.

Purpose of the Study:

  • To demonstrate transcriptomic analysis using single nuclei.
  • To assess the feasibility and reproducibility of single-nucleus RNA sequencing (snRNA-seq).
  • To explore the advantages of using nuclei over whole cells for transcriptomic studies.

Main Methods:

  • Isolation of single nuclei from mouse neural progenitor cell lines and hippocampal tissue.
  • Synthesis of double-stranded complementary DNA (cDNA) from single-nucleus RNA.
  • High-throughput sequencing and bioinformatic analysis of transcriptomic data.

Main Results:

  • Successful quantification of global gene expression from single nuclei.
  • Detection of over 16,000 protein-coding genes per nucleus on average.
  • Comparable gene expression variation between single nuclei and single cells.
  • Demonstrated technical reproducibility and excellent gene coverage.

Conclusions:

  • Single-nucleus transcriptomics is a viable method for analyzing gene expression.
  • Nuclei are easier to isolate from complex tissues like the central nervous system (CNS).
  • This method provides deeper insights into nuclear regulatory mechanisms and gene regulation at the single-cell level, avoiding bulk analysis limitations.