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Updated: May 5, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
An immobilized cell culture system for berberine production by Thalictrum minus cells
Y Kobayashi1, H Fukui, M Tabata
1Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida, Sakyo-ku, 606, Kyoto, Japan.
Abstract:
Thalictrum minus cells immobilized in calcium-alginate beads were cultured in a newly devised bioreactor for the purpose of producing berberine. This system could prevent cells from leaking out of the beads, allowing them to release most of the berberine synthesized into the liquid medium. Furthermore, the biosynthetic capability of the immobilized cells was found to be comparable to that of freely suspended cells.
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