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Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
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LC/MS and LC/MS/MS determination of protein tryptic digests

E C Huang1, J D Henion

  • 1Drug Testing and Toxicology, Cornell University, 925 Warren Drive, 14850, Ithaca, NY, USA.

Journal of the American Society for Mass Spectrometry
|November 20, 2013
PubMed
Summary

This study demonstrates online microbore liquid chromatography-mass spectrometry (LC/MS) for analyzing protein digests. This method accurately determines peptide molecular weights and provides sequence information for complex biological samples.

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