Related Experiment Video
Updated: May 5, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Published on: February 14, 2020
Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene
W H Chen1, K M Gartland, M R Davey
1Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, UK.
Abstract:
Sugarcane protoplasts were transformed to kanamycin resistance at a frequency of approximately 8 in 10(7) following PEG-induced uptake of Sma1 linearised pABD1 plasmid. DNA-treated protoplasts were cultured in agarose droplets, and protoplast-derived transformants selected on 80 μg ml(-1) kanamycin. Transformed tissues maintained on this level of antibiotic expressed APH(3')II activity, and contained DNA that hybridised to a probe with the APH(3')II gene.
Related Concept Videos
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Bacterial Transformation
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Transformation
Transgenic Organisms

