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Updated: May 5, 2026

Scalable Transfection of Maize Mesophyll Protoplasts
Published on: June 23, 2023
Transient expression of chimaeric genes in dividing and non-dividing cereal protoplasts after PEG-induced DNA uptake
1Max-Planck-Institut für Züchtungsforschung, Am Engelspfad, D-5000, Köln 30, Federal Republic of Germany.
Abstract:
Transient expression of chimaeric genes (neomycin phosphotransferase fused to four different promoters) was detected in suspension culture derived protoplasts of maize, barley and rice, in mesophyll protoplasts of maize, rice, rye, and root protoplasts of maize. The introduction and expression of foreign genes could be performed with both dividing and non-dividing protoplasts by applying the PEG transformation method. The significance of this method for the functional analysis of genes was demonstrated by the differential expression of a regulated gene in protoplasts of different tissues in agreement with its expression in the donor tissue.
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