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Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

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Published on: March 17, 2023

Transformation of lettuce (Lactuca sativa) mediated by Agrobacterium tumefaciens

R Michelmore¹, E Marsh, S Seely

¹Department of Vegetable Crops, University of California, 95616, Davis, CA, USA.

Plant Cell Reports

|November 20, 2013

Abstract:

Lactuca sativa can be routinely transformed using Ti plasmids of Agrobacterium tumefaciens containing a chimeric kanamycin resistance gene (NOS.NPTII.NOS). Critical experimental variables were plant genotype, bacterial concentration, presence of a nurse culture and timing of transfers between tissue culture media. Transformation was confirmed by the ability to callus and root in the presence of kanamycin, nopaline production, and by hybridization in Southern blots. Transformation has been achieved with several Ti vectors. Several hundred transformed plants have been regenerated. Kanamycin resistance was inherited monogenically. Homozygotes can be selected by growing R2 seedlings on media containing G418.

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