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Updated: May 5, 2026

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
Published on: August 20, 2011
Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.)
K Saka1, F R Katterman, J C Thomas
1University of Arizona, Forbes Building No. 36, Room 201, 85721, Tucson, AZ, USA.
Abstract:
Protoplasts were isolated from 12 day old subcultured phytohormone habituated callus tissue of Gossypium hirsutum L. (0.5% cellulysin-Calbiochem, 0.6% macerase-Calbiochem, 0.7M mannitol, and pH 5.0). After separation and purification (0.35M sucrose floatation medium), the protoplasts were cultured (K3 media of Kao et al., 1974 with 0.9 μM BAP, 5 μM IAA and 0.35M sucrose) in both liquid and solid medium at a density of 5×10(5) protoplasts/ml. Four weeks after isolation, cell regeneration and callus formation was observed.
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