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Updated: May 5, 2026

Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings GCMR in the Insect Antennal Lobe
Published on: February 24, 2013
NMR spectral assignment of lactone pheromone components emitted by Caribbean and Mexican fruit flies
1Agricultural Research Service U.S. Department of Agriculture, Insect Attractants, Behavior, and Basic Biology Research Laboratory, 32604, Gainesville, Florida.
Abstract:
By utilizing one- and two-dimensional nuclear magnetic resonance techniques, the complete assignments of the proton and carbon spectra of the lactones anastrephin, epianastrephin, and suspensolide have been accomplished. These compounds are pheromone components for both the Caribbean and Mexican fruit flies. The relative stereochemistries of anastrephin and epianastrephin were demonstrated by nuclear Overhauser difference spectroscopy. With complete spectral assignments now available, biosynthesis of these molecules may be studied by feeding specific isotopically labeled nutrients to flies and subsequently analyzing the volatiles produced for the presence and position of the labelling isotope.
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