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Related Experiment Video

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Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
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Alkaline phosphatase in boar sperm function.

D Bucci1, G Isani, E Giaretta

  • 1Dipartimento di Scienze Mediche Veterinarie (DIMEVET), Università degli Studi di Bologna, Ozzano dell'Emilia (BO), Italy.

Andrology
|November 20, 2013
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Summary

Alkaline phosphatase (AP) activity decreases during boar sperm capacitation. Supplementing with AP inhibits sperm capacitation and in vitro fertilization (IVF), suggesting AP maintains sperm quiescence before ejaculation.

Keywords:
IVFalkaline phosphatasesperm capacitationsperm cellssperm functionsperm quality parameters

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Area of Science:

  • Reproductive Biology
  • Enzymology
  • Biochemistry

Background:

  • Alkaline phosphatase (AP) is present in mammalian seminal plasma and spermatozoa.
  • The specific role of AP in male reproductive functions, particularly sperm capacitation and fertilization, remains unclear.

Purpose of the Study:

  • To investigate the function of alkaline phosphatase (AP) in boar sperm capacitation.
  • To evaluate the impact of AP on in vitro fertilization (IVF) and sperm parameters.

Main Methods:

  • Assayed AP activity in seminal plasma and spermatozoa (uncapacitated and in vitro capacitated).
  • Studied boar sperm capacitation in the presence of varying AP doses (1.2 and 2.5 IU/mL).
  • Evaluated sperm parameters (viability, acrosome integrity, capacitation status, tyrosine phosphorylation) and IVF outcomes.

Main Results:

  • High AP activity was found in seminal plasma and uncapacitated sperm, decreasing significantly after in vitro capacitation (IVC).
  • AP supplementation dose-dependently reduced acrosome integrity and capacitation status, and decreased tyrosine phosphorylation.
  • In vitro fertilization (IVF) showed a dose-dependent decrease in oocyte penetration with increased monospermic zygote rates.

Conclusions:

  • AP activity diminishes during sperm capacitation.
  • Exogenous AP addition during boar sperm capacitation suppresses the capacitation process and reduces fertilizing ability.
  • AP likely plays a role in maintaining sperm quiescence and modulating the acquisition of fertilizing capacity.