Detection of Oxytetracycline Residuein Infant Formulaby High-Performance Liquid Chromatography)HPLC(
Roya Khosrokhavar1, Mir-Jamal Hosseini, Shahram Shoeibi
1Food and Drug Laboratory Research center, Ministry of Health and Medical Education, Tehran, Iran. ; Food and Drug Control Laboratories, Ministry of Health and Medical Education, Tehran, Iran.
Insights
This study found no oxytetracycline (OTC) residues in infant formula, ensuring infant safety. HPLC analysis confirmed the absence of this common veterinary drug in various brands, safeguarding infant health.
Area of Science:
- Food safety analysis
- Analytical chemistry
- Pharmacology
Background:
- Drug residues in food, especially infant formula, pose toxicity risks, particularly for children.
- Oxytetracycline (OTC) is a broad-spectrum antibiotic used in animal husbandry, with potential toxicity and allergic reactions if present in infant formula.
- Previous research indicates High-Performance Liquid Chromatography (HPLC) is effective for detecting OTC in milk.
Purpose of the Study:
- To quantify residual oxytetracycline (OTC) levels in commercially available infant formulas.
- To assess the safety of infant formula regarding OTC contamination.
Main Methods:
- Utilized High-Performance Liquid Chromatography (HPLC) for sensitive detection of OTC.
- Established a Limit of Quantification (LOQ) of 51 ng/mL for the analytical method.
Main Results:
- No detectable residues of oxytetracycline (OTC) were found in any of the analyzed infant formula samples.
- All tested infant formulas from various manufacturers met safety standards concerning OTC.
Conclusions:
- Current infant formulas available on the market are free from oxytetracycline (OTC) contamination.
- The study confirms the safety of infant formulas concerning OTC residues, protecting vulnerable infants from potential adverse effects.
Abstract:
Determination of drug residues in food is of great importance due to their toxicity. Long-term exposure with low level of drug residues could be important, especially in children. Based on document study, oxytetracycline )OTC) is a prophylaxis and treatment agent for great number of diseases and possesses a broad spectrum activity against many pathogenic organisms and can be toxic or cause allergic reactions in some hypersensitive individual's if the residues of drug exist in the infant formula. The previous studies show that using high-performance liquid chromatography (HPLC) is useful for OTC detection in milk. Therefore, we decided to measure OTC in infant formula. The purpose of this study was to investigate residual OTC in consuming infant formula using HPLC. The limit of quantification (LOQ) was 51 ng/mL. This result showed that the samples had no residues of OTC in infant formula from different companies.
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