Related Experiment Video
Updated: May 5, 2026

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
Published on: March 1, 2016
Thermoresponsive oligo(ethylene glycol)-based polymer brushes on polymer monoliths for all-aqueous chromatography
1Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
Abstract:
Porous polymer monoliths onto which were grafted a thermoresponsive copolymer, poly(2-(2-methoxyethoxy)ethyl methacrylate (MEO2MA)-co-oligo(ethylene glycol) methacrylate (OEGMA)), were synthesized by the two-step atom transfer radical polymerization (ATRP) method. The copolymer-grafted monoliths were characterized by elemental analysis, scanning electron microscopy, and mercury intrusion porosimetry. They were further used as the thermoresponsive stationary phase for all-aqueous high-performance liquid chromatography (HPLC). The chromatograms of three steroids demonstrated that the chain length of the grafted copolymer, which was regulated by varying the grafting time, could affect the separation by providing different amounts of hydrophobic interaction sites with analytes. Additionally, the elution profiles of steroids on the stationary phase could also be tuned by the comonomer composition. The results showed that the porous polymer monoliths enabled separation of the test mixture in pure aqueous mobile phase under isocratic conditions. Furthermore, the proposed method provides a simple and promising tool in the design and construction of responsive surfaces for chromatography applications.
More Related Videos
09:34Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
Published on: September 20, 2016
13:57Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes
Published on: December 24, 2014
Related Concept Videos
Ion Exchange
Ion-Exchange Chromatography
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Analyte Adsorption and Distribution
Affinity Chromatography