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Related Experiment Videos

Factors affecting in vivo electrochemistry: electrode-tissue interaction and the ascorbate amplification effect.

H Echizen, C R Freed

    Life Sciences
    |July 7, 1986
    PubMed
    Summary

    Carbon paste electrodes change after brain implantation, affecting dopamine measurement. Ascorbate amplifies signals, improving detection but causing ambiguity in brain electrochemistry.

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    Area of Science:

    • Neuroscience
    • Analytical Chemistry
    • Electrochemistry

    Background:

    • In vivo electrochemistry is valuable for neuroscience but faces challenges in compound identification and quantification in brain extracellular fluid.
    • Controversy exists regarding the accuracy of measured dopamine concentrations using current electrochemical methods.

    Purpose of the Study:

    • To investigate alterations in carbon paste electrode performance after brain implantation.
    • To examine the influence of ascorbate on catecholamine detection and its implications for dopamine quantification.
    • To provide an explanation for potentially overestimated dopamine concentrations in brain extracellular fluid.

    Main Methods:

    • Carbon paste electrodes were temporarily implanted in the brain (20 min) and subsequently tested in vitro.

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  • Electrode sensitivity and oxidation potentials for dopamine, norepinephrine, serotonin, DOPAC, and 5-HIAA were analyzed.
  • The effect of varying ascorbate concentrations on catecholamine electrochemical signals was evaluated.

    • Main Results:

    • Brain-treated electrodes exhibited increased sensitivity and lower oxidation potentials for dopamine, norepinephrine, and serotonin.
    • A distinct peak for 3,4-dihydroxyphenylacetic acid (DOPAC) was observed, though with lower sensitivity compared to dopamine.
    • Ascorbic acid significantly amplified catecholamine peaks in a concentration-dependent manner, enhancing detectability but introducing ambiguity.
    • Ascorbate amplification of a 1 microM dopamine signal reached 4250% at physiological ascorbate concentrations (300 microM).

    Conclusions:

    • In vivo brain implantation alters carbon paste electrode characteristics, impacting neurochemical measurements.
    • Ascorbate significantly influences electrochemical signals, potentially leading to misinterpretation of dopamine and catecholamine concentrations in brain extracellular fluid.
    • The findings highlight the need for careful consideration of ascorbate's role in interpreting in vivo electrochemical data from the brain.