Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

9.1K
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
9.1K
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

14.2K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
14.2K
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

2.4K
2.4K
G-protein Coupled Receptors01:21

G-protein Coupled Receptors

92.3K
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
92.3K
G-protein Coupled Receptors01:21

G-protein Coupled Receptors

5.7K
5.7K
GTPases and their Regulation02:14

GTPases and their Regulation

7.9K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Comparison of the Efficacy and Safety of Modified Baerveldt Drainage Implants versus Ahmed Glaucoma Valves After 1 Year of Follow-Up.

Clinical ophthalmology (Auckland, N.Z.)·2026
Same author

IL-17A deficiency in HLA-DR3 transgenic mice enriches beneficial Prevotella species in gut to promote Tregs and reduce CNS autoimmunity.

Microbiome·2026
Same author

Urine-derived stem cell extracellular vesicles promote recovery from acute kidney injury in mice and human kidney organoids.

American journal of physiology. Renal physiology·2026
Same author

<i>hTERT</i> Expression, Regulation, and Prognostic Significance in Pediatric Medulloblastoma.

bioRxiv : the preprint server for biology·2026
Same author

Berzosertib enhances the sensitivity of pediatric diffuse midline glioma H3K27-altered cells to radiotherapy.

Cell death & disease·2026
Same author

Intratumoral plasma cells mediate CD8+ T cell infiltration and successful immune checkpoint blockade therapy in <i>de novo</i> MPNSTs.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: May 5, 2026

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
06:32

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

Published on: September 1, 2010

16.0K

G-protein coupled receptor expression patterns delineate medulloblastoma subgroups.

Kelsey L Whittier1, Erin A Boese, Katherine N Gibson-Corley

  • 1Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA 2524 JCP, USA. sue-odorisio@uiowa.edu.

Acta Neuropathologica Communications
|November 21, 2013
PubMed
Summary

G-protein coupled receptors (GPCRs) expression patterns in pediatric medulloblastoma reveal distinct tumor subgroups. This finding identifies novel GPCRs as potential therapeutic and imaging targets for this common childhood brain cancer.

More Related Videos

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
05:10

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice

Published on: October 3, 2010

11.6K
Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma
10:58

Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma

Published on: February 22, 2015

12.6K

Related Experiment Videos

Last Updated: May 5, 2026

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
06:32

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

Published on: September 1, 2010

16.0K
Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
05:10

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice

Published on: October 3, 2010

11.6K
Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma
10:58

Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma

Published on: February 22, 2015

12.6K

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Medulloblastoma is the most common pediatric malignant brain tumor, with four main genetic subgroups.
  • WNT and SHH pathways are well-defined drivers, but Groups 3 and 4 are less understood.
  • Molecular classification is crucial for diagnosis, staging, and therapy, yet druggable targets remain scarce.

Purpose of the Study:

  • To investigate G-protein coupled receptor (GPCR) expression patterns in medulloblastoma subgroups.
  • To identify distinct GPCRs that could serve as molecular targets for imaging and therapy.
  • To determine if GPCR expression can classify medulloblastoma subtypes.

Main Methods:

  • Analysis of GPCR expression patterns in medulloblastoma tumors.
  • Clustering of tumors based on GPCR expression profiles.
  • Validation of findings using an international dataset.

Main Results:

  • Medulloblastoma tumors formed distinct clusters based on GPCR expression, separate from normal cerebellum.
  • Two tumor clusters strongly correlated with the WNT and SHH medulloblastoma subgroups.
  • Specific GPCRs, such as LGR5 and GPR64 (WNT subgroup) and PTGER4 (SHH subgroup), were found to be differentially expressed.

Conclusions:

  • GPCR expression patterns can effectively classify medulloblastoma subgroups.
  • Identified GPCRs represent potential targets for medulloblastoma imaging and therapeutics.
  • Differential GPCR expression aids in understanding medulloblastoma tumorigenesis.