Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Genetic Variation

Genetic Variation

Genetic variation is the diversity in DNA sequences found among individuals of the same species. This diversity is crucial for a species' survival because it helps organisms adapt to environmental changes. Genetic variation begins with fertilization, where an egg and sperm cell merge. Each of these cells carries 23 chromosomes, up to 46 in the fertilized egg. Chromosomes are long DNA strands that contain genes, the basic units of heredity.
Genes exist in different versions called alleles,...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

Gene Evolution - Fast or Slow?

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...

Principles of Pharmacogenetics: Types of Genetic Variants

Principles of Pharmacogenetics: Types of Genetic Variants

The human genome is over 99.9% identical between individuals, yet genetic differences exist at millions of bases. The human genome contains approximately 3 million variant positions per individual, many of which are heterozygous, contributing to genetic diversity and individual traits. Genetic variations include single-nucleotide polymorphisms (SNPs), insertions, deletions, and copy number variations (CNVs).SNPs, the most common variation, involve single-base changes in DNA. These can be...

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

First Report of the Presence of East African Cassava Mosaic Virus in Cameroon.

Plant disease·2019

Same author

Regeneration of transgenic cassava plants (Manihot esculenta Crantz) through Agrobacterium-mediated transformation of embryogenic suspension cultures.

Plant cell reports·2019

Same author

Optimization of parameters for particle bombardment of embryogenic suspension cultures of cassava (Manihot esculenta Crantz) using computer image analysis.

Plant cell reports·2019

Same author

An efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants and axillary bud-derived meristems.

Plant cell reports·2019

Same author

The burden of chemotherapy-induced nausea and vomiting in children receiving hematopoietic stem cell transplantation conditioning: a prospective study.

Bone marrow transplantation·2017

Same author

Using Genomic Location and Coalescent Simulation to Investigate Gene Tree Discordance in Medicago L.

Systematic biology·2017

Search research articles

Related Experiment Video

Updated: May 5, 2026

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Ribosomal gene variation in soybean (Glycine) and its relatives.

J J Doyle¹, R N Beachy

¹L. H. Bailey Hortorium, Cornell University, 14853, Ithaca, NY, USA.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik

|November 21, 2013

Summary

Soybean ribosomal DNA (rDNA) shows consistent gene repeat structures across cultivated and wild varieties. Genetic analysis reveals significant variation in repeat length among wild Glycine species, but not within individual plants.

More Related Videos

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

Polysome Purification from Soybean Symbiotic Nodules

Polysome Purification from Soybean Symbiotic Nodules

Published on: July 1, 2022

Related Experiment Videos

Last Updated: May 5, 2026

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

Polysome Purification from Soybean Symbiotic Nodules

Polysome Purification from Soybean Symbiotic Nodules

Published on: July 1, 2022

Area of Science:

Plant genetics
Molecular evolution
Soybean biosystematics

Background:

Ribosomal RNA (rRNA) genes are crucial for protein synthesis and are organized in repeating units.
Understanding variation in these repeats is key to plant evolution and breeding.

Purpose of the Study:

To investigate variation in ribosomal RNA gene repeat length and restriction sites within the genus Glycine.
To compare the rDNA of cultivated soybean (Glycine max) with its wild relatives.

Main Methods:

Survey of 18S:25S ribosomal RNA gene repeats in soybean and related Glycine species.
Analysis of repeat length and restriction enzyme site polymorphisms.
rDNA mapping studies.

Main Results:

Significant differences in repeat size were observed among wild Glycine species, particularly in G. falcata.
Individual plants contained only a single type of repeat.
Cultivated soybean (Glycine max) and its wild progenitor (Glycine soja) exhibited identical rDNA sequences, with no variation found in over 40 accessions.
rDNA mapping data supported previous biosystematic findings and revealed new divergences.

Conclusions:

The ribosomal DNA of cultivated soybean and its wild progenitor are highly conserved.
Significant genetic diversity exists in rDNA repeats within wild Glycine species.
rDNA analysis provides valuable insights into plant evolutionary relationships and divergences.