Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

3.7K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.7K
Protein Networks02:26

Protein Networks

1.8K
1.8K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.1K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.1K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

1.0K
1.0K
Protein-protein Interfaces02:04

Protein-protein Interfaces

12.6K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.6K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

1.9K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vitro and in silico study of the endosulfan degradation by Bacillus subtilis sp. strain UAMC.

Biodegradation·2026
Same author

Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions.

Nature communications·2025
Same author

Moonlighting Proteins: Some Hypotheses on the Structural Origin of Their Multifunctionality.

International journal of molecular sciences·2025
Same author

Development of Texture-Modified Meat and Thickened Soup Combination for Oral Dysphagia Patients with Uniform Firmness and Solid Appearance.

Foods (Basel, Switzerland)·2025
Same author

Amino acids hydrophobic properties in proteins are derived from their atomic polarities.

European biophysics journal : EBJ·2025
Same author

The influence of the Debye-Hückel factor in estimating the distance between interacting monomers in self-assembling proteins.

European biophysics journal : EBJ·2025
Same journal

Cap 2'-O-methyltransferase CMTR2 regulates male meiosis independent of its methyltransferase activity.

Nucleic acids research·2026
Same journal

APE1 binds and processes abasic sites present in i-motif DNA and cooperates with PCBP1 in maintenance of telomeric stability.

Nucleic acids research·2026
Same journal

Acquisition of a novel restriction modification system regulates genetic flux and gene expression in the hypervirulent and globally disseminated CC17 lineage of group B Streptococcus.

Nucleic acids research·2026
Same journal

Trans-species microRNAs from the parasitic plant Cuscuta campestris specifically avoid loading onto self Argonautes.

Nucleic acids research·2026
Same journal

Neurochondrin promotes U5 snRNP maturation by regulating AAR2 release from PRPF8.

Nucleic acids research·2026
Same journal

Elongationless start-stop elements are stress-resilient translation gates that are more repressive than uTranslons.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: May 5, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

2.7K

MultitaskProtDB: a database of multitasking proteins.

Sergio Hernández1, Gabriela Ferragut, Isaac Amela

  • 1Departament de Bioquímica i Biologia Molecular, Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, Barcelona 08193, Spain,Laboratorio de Inmunología, Universidad de la República Regional Norte-Salto, Rivera 1350, Salto 50000, Uruguay and Departament de Medicina Experimental, Institut de Recerca Biomèdica, Universitat de Lleida, Lleida 25198, Spain.

Nucleic Acids Research
|November 21, 2013
PubMed
Summary
This summary is machine-generated.

Multitasking proteins, also known as moonlighting proteins, perform multiple biological functions. A new database, MultitaskProtDB, now catalogs over 288 such proteins, aiding research into cellular complexity.

More Related Videos

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances
07:35

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances

Published on: October 11, 2018

7.0K
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

7.4K

Related Experiment Videos

Last Updated: May 5, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

2.7K
Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances
07:35

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances

Published on: October 11, 2018

7.0K
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

7.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Bioinformatics

Background:

  • Proteins typically perform a single biological function.
  • Multitasking proteins (moonlighting proteins) execute two or more distinct functions.
  • Understanding moonlighting proteins is crucial for comprehending cellular complexity with a limited gene set.

Purpose of the Study:

  • To establish MultitaskProtDB, a centralized repository for multitasking proteins.
  • To facilitate the study and dissemination of data on moonlighting proteins.
  • To provide a user-friendly web interface for accessing information on these proteins.

Main Methods:

  • Compilation of data from scientific literature.
  • Development of a web-based database (MultitaskProtDB).
  • Inclusion of protein identifiers (NCBI, UniProt), functions, structural states, and references.

Main Results:

  • MultitaskProtDB contains over 288 multitasking proteins.
  • The database includes accession numbers, biological functions, monomeric/oligomeric states, and PDB codes.
  • Data allows for analysis of function pairs, phylogenetic conservation, and other protein characteristics.

Conclusions:

  • MultitaskProtDB addresses the lack of a dedicated database for moonlighting proteins.
  • The database serves as a valuable resource for researchers studying protein multifunctionality.
  • Facilitates insights into the prevalence and characteristics of multitasking proteins in biological systems.