Related Experiment Video
Updated: May 5, 2026
Measuring the Osmotic Water Permeability Coefficient Pf of Spherical Cells: Isolated Plant Protoplasts as an Example
Published on: October 8, 2014
Apparent free space and cell volume estimation: A non-destructive method for assessing the growth and membrane
A J Parr1, J I Smith, R J Robins
1Agricultural and Food Research Council, Food Research Institute, Colney Lane, NR4 7UA, Norwich, UK.
Abstract:
Pre-existing methods for measuring cell or organelle volume based on the selective permeability of biological membranes have been modified to make them suitable for determining the intracellular volume of immoblised cells. When a freely permeable substance (e.g. tritiated water) and an impermeable substance ((14)C labelled mannitol is often suitable) are mixed with an immobilised cell culture, the two substances are diluted to different degrees. The extent of the difference allows the total intracellular volume of intact cells to be calculated. This volume is shown to be a useful parameter for assessing cell growth. The application of the method to follow membrane integrity and cell viability is also discussed.
More Related Videos
10:26AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions
Published on: July 24, 2014
09:11High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices
Published on: March 26, 2018
Related Concept Videos
01:23Microbial Growth Measurement: Direct Methods
01:27Microbial Growth Measurement: Indirect Methods