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A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
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Improved efficiency of plant regeneration from protoplasts of eggplant Solanum melongena L
1Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, 50 250, Bet Dagan, Israel.
Plant Cell Reports
|November 21, 2013
Abstract:
Eggplant (Solanum melongena L.) mesophyll protoplasts were obtained from in vitro growing plants of line 410 and cv. 'Classic'. Relatively high (15%) plating efficiency was achieved using petri dishes with alternate quadrants containing reservoir medium (R medium + 1% activated charcoal) and culture medium. Shoot regeneration occurred within 6 weeks following initiation of protoplast culture.
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