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Production of chrysanthemic acid and pyrethrins by tissue cultures ofChrysanthemum cinerariaefolium
J S Kueh1, I A Mackenzie, G Pattenden
1Department of Chemistry, The University, NG7 2RD, Nottingham, UK.
Abstract:
Tissue cultures ofChrysanthemum cinerariaefolium were established, and then used to study the production of pyrethrin insecticides, and their precursor chrysanthemic acid. Callus cultures and root-differentiated cultures did not contain pyrethrins whereas shoot differentiated callus was found to produce the pyrethrins. Chrysanthemic acid was isolated by extraction from callus cultures, and feeding(14)C-labelled chrysanthemic acid to a cell suspension ofC. cinerariaefolium established that the acid accumulates largely as a glucoside ester.
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