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Technique for using microencapsulated terpenes in lepidopteran artificial diets.

K M Clancy1, R D Foust, T G Huntsberger

  • 1Forestry Sciences Laboratory, Rocky Mountain Forest and Range Experiment Station, 86001, Flagstaff, Arizona.

Journal of Chemical Ecology
|November 21, 2013
PubMed
Summary

We developed microencapsulation for terpene essential oils, improving delivery in insect diets. This technique aids studies on plant-herbivore interactions by reducing terpene volatilization.

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Area of Science:

  • Chemical Ecology
  • Entomology
  • Materials Science

Background:

  • Terpenes are vital plant compounds mediating herbivore interactions.
  • Terpenes in artificial diets often volatilize, limiting their effectiveness in studies.
  • Douglas fir (Pseudotsuga menziesii) oleoresin contains various terpenes crucial for its defense.

Purpose of the Study:

  • To develop and test a microencapsulation technique for terpene compounds.
  • To assess the impact of microencapsulated terpenes on western spruce budworm (Choristoneura occidentalis) survival and reproduction.
  • To provide a method for delivering terpenes in a manner that mimics their natural state in plant foliage.

Main Methods:

  • Gelatin-walled microcapsules were formed around eight common Douglas fir terpenes.
  • Microencapsulated terpenes were incorporated into artificial diets for bioassays.
  • A three-generation bioassay was conducted with western spruce budworm (Choristoneura occidentalis) larvae.

Main Results:

  • The microencapsulation technique successfully packaged terpene essential oils.
  • Preliminary bioassays confirmed that western spruce budworm (C. occidentalis) larvae consumed diets containing microencapsulated terpenes.
  • The method reduces terpene volatilization from artificial diets.

Conclusions:

  • Microencapsulation offers a viable method for delivering terpenes in insect diet studies.
  • This technique enhances the study of plant-herbivore interactions by improving terpene stability and delivery.
  • The method is applicable to laboratory, greenhouse, and field experiments investigating terpene functions.