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Identification of calmodulin in the green alga Mougeotia and its possible function in chloroplast reorientational
G Wagner1, P Valentin, P Dieter
1Botanisches Institut I der Justus-Liebig-Universität, Senckenbergstrasse 17-21, D-6300, Giessen, Germany.
Abstract:
A soluble protein was isolated from Mougeotia by chloropromazine-sepharose 4 B affinity chromatography. The protein matches the properties of calmodulin in terms of heat stability, Ca(2+)-dependent electrophoretic mobility in sodium-dodecyl-sulfate polyacrylamide gels, and its ability to activate cyclic nucleotide phosphodiesterase in a Ca(2+)-dependent manner. Phytochrome-mediated chloroplast reorientational movement in Mougeotia was inhibited by the calmodulin antagonist trifluoperazine, a hydrophobic compound, or N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a hydrophilic compound; 50% inhibition (IC50) of chloroplast movement is caused by 20-50 μmol l(-1) trifluoperazine or 100 μmol l(-1) W-7. The Ca(2+)-calmodulin may act as an intermediate in the chloroplast reorientational response in Mougeotia governed by phytochrome.
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