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Related Concept Videos

Overview of Skeletal Muscle01:15

Overview of Skeletal Muscle

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Skeletal muscles are composed of a bundle of muscle fibers and are attached to bones through tendons. Each skeletal muscle fiber is a single muscle cell. The sarcolemma, the plasma membrane of a skeletal muscle cell, consists of a lipid bilayer and glycocalyx that supports muscle fibers. The sarcolemma extends into the muscle cells to form tubular structures called transverse or T-tubules. Each side of the T-tubules consists of a membrane-bound structure called the sarcoplasmic reticulum,...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Disorders of the Skeletal Muscle01:28

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The clinical conditions affecting the skeletal muscle tissue are broadly categorized as musculoskeletal and neuromuscular disorders.
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Skeletal muscle is the most abundant type of muscle in the body. Tendons are the connective tissue that attaches skeletal muscle to bones. Skeletal muscles pull on tendons, which in turn pull on bones to carry out voluntary movements.
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Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
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Related Experiment Video

Updated: May 5, 2026

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis
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Comparative gene expression profiling reveals key pathways in septic skeletal muscle.

H-Z Xu1, M-T Wang, B Mei

  • 1Department of Emergency, Changhai Hospital, Second Military Medical University, Shanghai, China. hejiansmmu@126.com

European Review for Medical and Pharmacological Sciences
|November 21, 2013
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Summary

Sepsis significantly alters skeletal muscle gene expression, impacting pathways like insulin signaling and apoptosis. This research reveals key molecular changes in sepsis-induced muscle dysfunction, aiding in developing new therapies.

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Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses
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Area of Science:

  • Molecular biology
  • Genomics
  • Sepsis research

Background:

  • Sepsis frequently leads to skeletal muscle dysfunction.
  • The molecular mechanisms driving this dysfunction remain incompletely understood.

Purpose of the Study:

  • To compare the skeletal muscle transcriptome of sepsis patients with controls.
  • To identify molecular mechanisms underlying sepsis-induced skeletal muscle dysfunction.

Main Methods:

  • Downloaded and analyzed Gene Expression Omnibus (GEO) dataset GSE13205 (13 sepsis, 8 control samples).
  • Identified differentially expressed genes (DEGs) using t-tests.
  • Constructed a transcriptional regulatory network and performed pathway enrichment analysis (DAVID).
  • Retrieved relevant small molecules using Connectivity Map.

Main Results:

  • Identified 287 DEGs (149 upregulated, 138 downregulated) in sepsis.
  • Constructed a transcriptional regulatory network with 83 nodes and 98 edges, identifying 5 key transcription factors.
  • Significantly altered pathways included insulin signaling, neurotrophin signaling, fructose/mannose metabolism, circadian rhythm, and apoptosis.
  • Identified potential therapeutic molecules like trazodone and thapsigargin.

Conclusions:

  • The study provides insights into molecular changes in sepsis-related skeletal muscle dysfunction.
  • Findings may aid in understanding sepsis pathogenesis and developing targeted therapies.