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Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
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Pixe analysis of reproductive fluids.

G S Hall1, R M Shelden

  • 1Dept. of Chemistry, Rutgers, The State University of New Jersey, New Brunswick, 08903, USA.

Biological Trace Element Research
|November 21, 2013
PubMed
Summary
This summary is machine-generated.

Proton-induced X-ray emission quantified trace elements in human follicular fluid and spermatozoa. This elemental analysis of sperm may approximate the femtogram quantities of trace elements transferred during fertilization.

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Area of Science:

  • Biomedical science
  • Analytical chemistry
  • Reproductive biology

Background:

  • Elemental composition analysis is crucial for understanding biological processes.
  • Human follicular fluid and spermatozoa play key roles in fertilization.
  • Trace elements are vital for cellular function and reproductive health.

Purpose of the Study:

  • To quantitatively determine elemental composition in human follicular fluid and spermatozoa using proton-induced X-ray emission (PIXE).
  • To investigate interelement correlations within these biological samples.
  • To explore the potential of spermatozoa elemental analysis for estimating trace element transfer during fertilization.

Main Methods:

  • Utilized a 3.8-MeV proton beam for PIXE analysis.
  • Analyzed human follicular fluid (100-mg pellets) and spermatozoa (4-8 mg pellets).
  • Quantitatively determined elements including S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, and Br.

Main Results:

  • Identified and quantified multiple elements in both follicular fluid and spermatozoa.
  • Observed high interelement Spearman correlation coefficients in both sample types.
  • Found correlations between elemental concentrations and clinical parameters.
  • Demonstrated that elemental quantities in spermatozoa are in the femtogram range.

Conclusions:

  • PIXE is effective for multielemental analysis of human follicular fluid and spermatozoa.
  • Elemental profiles of spermatozoa show significant interelement correlations.
  • Spermatozoa elemental analysis offers a method to approximate trace element quantities transferred to the ooplasm during fertilization.