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Updated: May 5, 2026

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Elemental microanalysis of individual blood cells.

P M O'Brien¹, G J Legge

¹School of Physics, University of Melbourne, 3052, Parkville, Victoria, Australia.

Biological Trace Element Research

|November 21, 2013

Summary

Researchers used a scanning proton microprobe to analyze red blood cells, revealing reproducible elemental distributions and visualizing cell structure. This technique offers a detailed look at trace elements within individual cells.

Area of Science:

Biophysics
Analytical Chemistry
Hematology

Background:

Red blood cells (RBCs) are crucial for oxygen transport.
Understanding elemental composition of RBCs is vital for diagnosing and treating various diseases.
Previous methods lacked the spatial resolution to analyze elemental distribution within single RBCs.

Purpose of the Study:

To investigate the elemental composition and spatial distribution of trace elements in single, freeze-dried red blood cells.
To assess the reproducibility and reliability of elemental analysis using a scanning proton microprobe.
To visualize the three-dimensional structure of red blood cells based on elemental mapping.

Main Methods:

Utilized a scanning proton microprobe for high-resolution elemental analysis.

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Employed Particle-Induced X-ray Emission (PIXE) and proton scattering techniques.

Analyzed freeze-dried, whole-blood specimens at a spatial resolution of 1.5 μm.

Main Results:

Achieved reproducible trace elemental spectra for individual red blood cells.
Confirmed no significant elemental loss during proton beam irradiation.
Generated quantitative two- and three-dimensional elemental maps (H, C, P, S, Cl, K, Fe).
Visualized the characteristic biconcave disk shape of red blood cells.

Conclusions:

Scanning proton microprobe analysis is a reliable method for studying elemental composition in single red blood cells.
The technique provides high spatial resolution for mapping elements and visualizing cellular morphology.
This approach has potential applications in hematological research and diagnostics.