Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correspondence: Caution May Be Required with mRNA Covid-19 Vaccinations Related to the Corneal Endothelium.

Ophthalmic epidemiology·2026
Same author

Metaproteomic profiling reveals viral proteins and associated host proteomic alterations in glioblastoma.

Scientific reports·2026
Same author

Assessment of visual contrast sensitivity in biotoxin-exposed individuals using four testing methods.

Annals of medicine·2026
Same author

Australasian consensus recommendations on stem cell therapies for managing ocular surface diseases.

The ocular surface·2026
Same author

In situ spatial transcriptomics reveals novel markers of the limbal stem cell niche and ocular surface epithelia.

Stem cell reports·2026
Same author

Retinal glial cells in glaucoma and age-related retinal diseases: Inflammatory responses, disease transitions, and translational perspectives.

Neural regeneration research·2026

Related Experiment Video

Updated: May 5, 2026

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
10:26

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

29.0K

Detecting human papillomavirus in ocular surface diseases.

Mark Woods1, Sharron Chow, Benjamin Heng

  • 1School of Medical Sciences, University of New South Wales, Sydney, Australia.

Investigative Ophthalmology & Visual Science
|November 21, 2013
PubMed
Summary

Human papillomavirus (HPV) was not found in pterygia but was detected in 6.5% of ocular-surface squamous neoplasia (OSSN), specifically squamous cell carcinoma of the conjunctiva. This suggests HPV-16 acts as a cofactor, not a direct cause, in OSSN development.

Keywords:
conjunctivaepitheliumneoplasiaocular surfacepterygiavirus

More Related Videos

Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia
06:15

Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia

Published on: August 9, 2024

1.7K
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

11.4K

Related Experiment Videos

Last Updated: May 5, 2026

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
10:26

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

29.0K
Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia
06:15

Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia

Published on: August 9, 2024

1.7K
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

11.4K

Area of Science:

  • Ophthalmology
  • Virology
  • Oncology

Background:

  • Human papillomavirus (HPV) is suspected to contribute to ocular surface diseases like pterygia and OSSN.
  • Previous studies report conflicting HPV prevalence, necessitating standardized detection methods.

Purpose of the Study:

  • To determine HPV prevalence in pterygia and OSSN.
  • To establish a standardized method for detecting HPV DNA in ocular tissues.

Main Methods:

  • DNA extraction from various ocular specimens (n=160).
  • Nested PCR with consensus and subtype-specific primers for HPV detection.
  • Confirmatory assays including sequencing, histology, and immunohistochemistry for HPV E6 and p16.

Main Results:

  • HPV was absent in pterygia and normal conjunctiva.
  • HPV was detected in 6.5% of OSSN samples, specifically in 12.5% of conjunctival squamous cell carcinomas (SCCC).
  • HPV-16 was identified in all positive cases, correlating with koilocytes and p16 expression. No association with EBV or CMV was found.

Conclusions:

  • The low prevalence of HPV-16 in ocular surface disease indicates it is a cofactor rather than a direct cause.
  • Standardized detection methods are crucial for accurate HPV assessment in ocular pathologies.