Blocked autophagy enhances radiosensitivity of nasopharyngeal carcinoma cell line CNE-2 in vitro
Yusheng Wang1, Wanzhong Yin, Xuewei Zhu
1Department of Otolaryngology Head & Neck Surgery, First Hospital of Jilin University , Changchun , China.
Conclusion:
Autophagy was involved in the radiation treatment of CNE-2 cells, and blocked autophagy enhances the radiosensitivity of nasopharyngeal carcinoma cell line CNE-2 in vitro.
Objective:
To determine whether autophagy induced by radiation therapy contributes to tumor cell death or represents a mechanism of resistance to therapy-mediated cell death.
Methods:
Autophagy in the CNE-2 nasopharyngeal carcinoma cells after radiation treatment was determined by quantitative GFP-LC3 analysis, electron microscopy, and autophagy-related molecules analysis by Western blotting. The contribution of autophagy to the cell viability was determined by MTT assay and clonogenic assay.
Results:
Autophagy was involved in CNE-2 cells post radiation treatment, and autophagy could ameliorate the cell viability post radiation. On the other hand, inhibition of autophagy could reduce cell viability and decrease the cell survival.
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