Metabolite profiling and network analysis reveal coordinated changes in grapevine water stress response
Uri Hochberg, Asfaw Degu, David Toubiana
1the French Associates Institute for Agriculture and Biotechnology of Drylands (FAAB), the Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, 84990 Sede Boqer, Israel. fait@bgu.ac.il.
BMC Plant Biology
|November 22, 2013
Summary
Grapevine water deficit triggers common metabolic shifts, particularly in nitrogen metabolism, affecting amino acids and organic acids. Shiraz shows more intense changes than Cabernet Sauvignon, highlighting cultivar-specific stress responses.
Area of Science:
- Plant Physiology
- Plant Metabolism
- Drought Stress Response
Background:
- Investigated grapevine (Vitis vinifera) metabolic responses to water deficit.
- Focused on two cultivars, Shiraz and Cabernet Sauvignon, with differing hydraulic behaviors.
Purpose of the Study:
- To elucidate cultivar-specific metabolic alterations under water deficit.
- To understand the role of central and specialized metabolism in grapevine stress tolerance.
- To identify key metabolites involved in grapevine drought response.
Main Methods:
- Comparative analysis of leaf water potential and metabolic profiles (primary and specialized).
- Principal Component Analysis (PCA) for metabolic pattern discrimination.
- Phloem sap analysis for abscisic acid (ABA) and benzoate derivatives.
- Correlation-based network analysis to assess metabolic connectivity.
Main Results:
- Water deficit induced higher osmolality and lower C/N ratios in both cultivars, with more pronounced effects in Shiraz.
- Significant increases in amino acids (e.g., Proline, Valine) and changes in phenylpropanoid pathways were observed.
- Phloem sap ABA and benzoate levels increased under drought, correlating with reduced stomatal conductance and suberinization.
- Shiraz exhibited greater metabolic network connectivity and coordinated stress responses compared to Cabernet Sauvignon.
Conclusions:
- Vitis vinifera cultivars share common metabolic strategies for water deficit response, with nitrogen metabolism being crucial.
- Cabernet Sauvignon displayed milder metabolic changes, suggesting more efficient stomatal regulation under stress.
- Network analysis effectively identified key metabolites (e.g., Proline, Quercetin, Ascorbate) with significant roles in grapevine stress response.
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