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Related Concept Videos

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.5K

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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
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Pre-analytical and analytical variability in absolute quantitative MRM-based plasma proteomic studies.

Andrew J Percy1, Carol E Parker, Christoph H Borchers

  • 1University of Victoria-Genome British Columbia Proteomics Centre, 3101-4464 Markham St, Victoria, BC V8Z 5N3, Canada.

Bioanalysis
|November 22, 2013
PubMed
Summary

Quantitative plasma proteomics, using targeted multiple reaction monitoring mass spectrometry (MRM-MS) and labeled standards, requires standardized workflows. This review details factors affecting precision and accuracy for reliable protein biomarker discovery.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biomedical Science

Background:

  • Quantitative plasma proteomics is increasingly vital for biological and biomedical research.
  • High precision and accuracy are crucial for translating findings into clinical applications, especially in protein biomarker discovery.

Purpose of the Study:

  • To review pre-analytical and analytical variables impacting the precision and accuracy of absolute quantitative plasma proteomics.
  • To highlight strategies for minimizing variability and identify areas for future research.

Main Methods:

  • Discussion of targeted multiple reaction monitoring mass spectrometry (MRM-MS) with isotopically labeled standards.
  • Review of pre-analytical and analytical workflow stages affecting quantitative measurements.

Main Results:

  • Identified key variables in plasma proteomic workflows that influence measurement precision and accuracy.
  • Proposed strategies to mitigate variability in quantitative proteomic analyses.

Conclusions:

  • Standardization of procedures and performance evaluation are imperative for reproducible and transferable quantitative plasma proteomics.
  • Addressing identified unmet needs will advance the clinical utility of protein biomarker research.