Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

7.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
7.5K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

2.6K
2.6K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

1.9K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
1.9K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.5K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.5K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.1K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.1K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

23.0K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
23.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring the Role of Macrophage Marker CD68 in Pediatric Acute Myeloid Leukemia.

International journal of molecular sciences·2026
Same author

Profiling histone post-translational modifications to identify signatures of epigenetic drug response in T-cell acute lymphoblastic leukemia.

Scientific reports·2026
Same author

In Memoriam: Dmitri Krysko.

Apoptosis : an international journal on programmed cell death·2026
Same author

Decoding NOTCH1: From T-Cell Development Guardian to Driver of Pediatric T-Cell Lymphoblastic Lymphoma.

International journal of molecular sciences·2026
Same author

Targeting replication stress in neuroblastoma by exploiting the synergistic potential of second generation RRM2 and CHK1 inhibitors.

Cell death & disease·2026
Same author

Age and surgical complexity outperform preoperative Geriatric-8 for 30-day major complications in older head and neck cancer.

Oral oncology·2025

Related Experiment Video

Updated: May 5, 2026

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

8.5K

Long non-coding RNAs in pluripotent stem cell biology.

Tim Lammens1, Inge D'hont, Katharina D'Herde

  • 1a Department of Pediatric Hematology-Oncology and Stem Cell Transplantation , Ghent University Hospital, 3K12D , De Pintelaan 185, 9000 Ghent , Belgium.

The Veterinary Quarterly
|November 22, 2013
PubMed
Summary

Long non-coding RNAs are crucial regulators of pluripotent stem cells, controlling their self-renewal and differentiation. These molecules offer new insights into tissue regeneration and maintaining pluripotency.

More Related Videos

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

11.1K
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.1K

Related Experiment Videos

Last Updated: May 5, 2026

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

8.5K
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

11.1K
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.1K

Area of Science:

  • Stem cell biology
  • Molecular biology
  • Genomics

Background:

  • Pluripotent stem cells possess unlimited self-renewal and differentiation potential.
  • Key factors regulating pluripotency induction and maintenance are being identified.
  • Long non-coding RNAs (lncRNAs) are emerging as significant regulators in this field.

Purpose of the Study:

  • To review recent findings on the role of lncRNAs in regulating pluripotency.
  • To highlight lncRNAs as potential markers for pluripotent stem cells.
  • To discuss the implications of lncRNAs in tissue regeneration.

Main Methods:

  • Literature review of recent research on lncRNAs and pluripotency.
  • Analysis of studies investigating lncRNA functions in stem cell regulation.
  • Synthesis of current knowledge on lncRNA roles as regulators and markers.

Main Results:

  • lncRNAs are confirmed key regulators of pluripotent stem cell identity.
  • Specific lncRNAs are associated with maintaining or disrupting pluripotency.
  • lncRNAs show potential as biomarkers for assessing stem cell states.

Conclusions:

  • lncRNAs play critical roles in governing stem cell pluripotency.
  • Understanding lncRNA functions advances knowledge of stem cell biology.
  • lncRNAs hold promise for therapeutic applications in tissue regeneration.