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Updated: May 5, 2026

Automated Analysis of C. elegans Fluorescence Images using SegElegans
Published on: October 10, 2025
An evolutionary approach for image segmentation
Alessia Amelio1, Clara Pizzuti
1Institute for High Performance Computing and Networking (ICAR), National Research Council of Italy (CNR), Via P. Bucci 41C, 87036 Rende, Italy amelio@icar.cnr.it.
Abstract:
The paper explores the use of evolutionary techniques in dealing with the image segmentation problem. An image is modeled as a weighted undirected graph, where nodes correspond to pixels, and edges connect similar pixels. A genetic algorithm that uses a fitness function based on an extension of the normalized cut criterion is proposed. The algorithm employs the locus-based representation of individuals, which allows for the partitioning of images without setting the number of segments beforehand. A new concept of nearest neighbor that takes into account not only the spatial location of a pixel, but also the affinity with the other pixels contained in the neighborhood, is also defined. Experimental results show that our approach is able to segment images in a number of regions that conform well to human visual perception. The visual perceptiveness is substantiated by objective evaluation methods based on uniformity of pixels inside a region, and comparison with ground-truth segmentations available for part of the used test images.

