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Opportunities for live cell FT-infrared imaging: macromolecule identification with 2D and 3D localization
Eric C Mattson1, Ebrahim Aboualizadeh, Marie E Barabas
1Department of Physics, University of Wisconsin-Milwaukee, 1900 E Kenwood Blvd, Milwaukee, WI 53211, USA. cjhirsch@uwm.edu.
International Journal of Molecular Sciences
|November 22, 2013
Summary
Fourier transform infrared (FTIR) spectromicroscopy now enables live-cell chemical imaging. This review covers advances in FTIR technology for studying cellular structure and chemistry in real-time.
Area of Science:
- Biology
- Medicine
- Chemical Imaging
Background:
- Infrared (IR) spectromicroscopy is an evolving chemical imaging technique with potential in biology and medicine.
- Recent technological advancements have enabled diffraction-limited Fourier transform infrared (FTIR) imaging of living cells.
Purpose of the Study:
- To review advances in FTIR spectromicroscopy for live-cell imaging.
- To highlight the utility of FTIR for studying cellular chemistry and structure.
Main Methods:
- Review of recent developments in IR sources, detectors, and measurement techniques.
- Focus on Fourier transform infrared (FTIR) spectromicroscopy.
Main Results:
- Diffraction-limited FTIR imaging of living cells is now achievable.
- Rapid, non-destructive measurements are facilitated by new IR sources and detectors.
- Advancements enable 2D and 3D IR imaging of live cells.
Conclusions:
- FTIR spectromicroscopy is a powerful tool for real-time analysis of cellular chemistry.
- The technique offers significant utility for biological and medical research on living cells.
