Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

88.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dopamine replacement therapy modulates DDC, prolactin and AOC3 levels in Parkinson's disease.

NPJ Parkinson's disease·2026
Same author

Patient cerebral organoids capture Alzheimer's disease proteomic biomarkers and drug targets.

bioRxiv : the preprint server for biology·2026
Same author

Transmission of rifampicin-resistant tuberculosis in Ho Chi Minh City, Viet Nam: a prospective genomic epidemiology study.

The Lancet regional health. Western Pacific·2026
Same author

A highly efficient method to differentiate CGRP-expressing peptidergic nociceptors from human induced pluripotent stem cells.

Stem cell reports·2026
Same author

The embryonic origins of site-specific arthritis.

Nature immunology·2026
Same author

Single-cell and spatial transcriptomic analyses of gene therapy-associated retinal inflammation in non-human primates.

Molecular therapy. Advances·2026
Same journal

Editorial Expression of Concern: Hindering the biofilm of microbial pathogens and cancer cell lines development using silver nanoparticles synthesized by epidermal mucus proteins from Clarias gariepinus.

BMC biotechnology·2026
Same journal

Computer-guided enzyme engineering of PET hydrolase mutants towards improved PET affinity.

BMC biotechnology·2026
Same journal

Synthesis and application of exopolysaccharide-selenium nanocomposite produced by Pseudoduganella armeniaca ZMN-3.

BMC biotechnology·2026
Same journal

Read-mapping signatures for molecular characterization of transgenic DNA insertions in paired-end Illumina data.

BMC biotechnology·2026
Same journal

Comparative analysis of MinION and MiSeq using 16S rRNA gene amplicon sequencing in human gut microbiome.

BMC biotechnology·2026
Same journal

Integrated structure-based and systems-level identification of PDE9A inhibitors for heart failure with preserved ejection fraction.

BMC biotechnology·2026
See all related articles

Related Experiment Video

Updated: May 5, 2026

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

9.9K

Improved workflows for high throughput library preparation using the transposome-based Nextera system.

Sarah Lamble1, Elizabeth Batty, Moustafa Attar

  • 1Wellcome Trust Centre for Human Genetics, OX3 7BN Oxford, UK. slamble@well.ox.ac.uk.

BMC Biotechnology
|November 22, 2013
PubMed
Summary
This summary is machine-generated.

This study optimized the Nextera library preparation protocol for Illumina sequencing, improving throughput and reducing costs. A new workflow enhances sample purity and allows more samples per sequencing run.

More Related Videos

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

9.8K
gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

11.7K

Related Experiment Videos

Last Updated: May 5, 2026

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

9.9K
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

9.8K
gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

11.7K

Area of Science:

  • Genomics
  • Molecular Biology
  • Next-Generation Sequencing

Background:

  • The Nextera protocol for Illumina sequencing requires pure DNA, accurate quantification, and column cleanup, limiting high-throughput applications.
  • These limitations hinder rapid sample preparation and increase reagent costs.

Purpose of the Study:

  • To develop a robust and cost-effective workflow for Nextera library preparation.
  • To address bottlenecks in the Nextera protocol for high-throughput and rapid sample preparation.

Main Methods:

  • Implemented an initial bead-based normalization step to eliminate the need for DNA quantification.
  • Developed a low-volume modified protocol and tested its robustness across genomes with varying GC content.
  • Designed custom index tags and primers to increase multiplexing capacity on Illumina instruments.

Main Results:

  • The bead-based normalization improved sample purity and removed the requirement for input DNA quantification.
  • A 75% cost reduction was achieved with the modified low-volume protocol.
  • Custom index tags and primers enabled simultaneous sequencing of more samples per Illumina lane.

Conclusions:

  • The modified Nextera protocol overcomes previous limitations, enabling reproducible, high-throughput library construction.
  • This optimized workflow harnesses the full potential of the Nextera kit while significantly reducing associated costs.