Two dimensional analysis of thylakoid membrane polypeptides of a barley mutant

One dimensional SDS-PAGE and two dimensional electrophoresis of the thylakoid membrane proteins of a barley mutant and its original variety were carried out. It was found that (1) approximately 20 bands could be identified. The mutant lacked one band at 23 kd, and two bands at 17.5 nd 18.3 kd were fainter when compared with the original variety which served as the standard. (2) In two dimensional patterns the 23 kd band was resolved into two faint bands which were composed of three peptide spots having a pH of 6.35, 6.46 and 6.50, respectively. The band in the 17-18 kd region was resolved into five heavy peptide spots with varying pH values of 6.35-6.62.