Somatic embryogenesis from mesophyll protoplasts of Trigonella corniculata (leguminosae)
D Y Lu1, M R Davey, E C Cocking
1Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, Nottingham, UK.
Plant Cell Reports
|November 22, 2013
Abstract:
Mesophyll protoplasts isolated enzymatically from Trigonella corniculata divided to form callus, with a plating efficiency of 49% in Kao (1977) medium. Protoplast-derived tissues formed somatic embryoids at high frequency on MS medium with 2.0 mg L(-7) NAA and 0.5 mg L(-7) BAP. Embryoids developed into plants on MS medium lacking hormones.
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