Artifical gynogenesis and mapping of gene-centromere distances in the paradise fish, Macropodus opercularis

An efficient method has been developed for the production of gynogenetic paradise fish in order to obtain genetically homogeneous strains in a relatively short period. Diploidy of the gynogenetic embryos was restored by inhibition of the second meiotic division, and consecutive generations of normal, fully viable offspring were reared. Crossing-over frequencies and the relative positions of four marker loci were determined by analysing electrophoretic patterns of serum transferrin and esterases in the gynogenetic progeny of heterozygous females.