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Chromosomal assignment of genes coding for the wheat gliadin protein components of the cultivars 'Cheyenne' and
D Lafiandra1, D D Kasarda, R Morris
1US Department of Agriculture, Food Proteins Research Unit, Western Regional Research Center, Agricultural Research Service, 94710, Berkeley, CA, USA.
Abstract:
The gliadin proteins of the hexaploid bread wheat cultivar 'Cheyenne' (Triticum aestivum L. var. aestivum), which has good mixing and baking characteristics, were analyzed by 2-dimensional (2-pH) polyacrylamide gel electrophoresis (pH 3.2 in the 1st dimension, pH 9.2 in the 2nd). Genes for most of the 35 resolved components were assigned to the chromosomes of homoeologous groups 1 and 6 through the use of various aneuploids and substitution lines. A similar analysis was carried out for the cultivar 'Chinese Spring', which does not have good quality, and which had been analyzed by a different 2-dimensional procedure by Wrigley and Shepherd (Ann NY Acad Sci 209:154, 1973). A coordinate system was devised for description of the components in the 2-dimensional patterns.
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