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Shoot regeneration from mesophyll protoplasts and leaf explants of Rehmannia glutinosa
1Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, UK.
Abstract:
Mesophyll protoplasts obtained from leaves of shoot cultures of Rehmannia glutinosa were cultured in Murashige and Skoog (1962) liquid or liquid-over-agar medium containing 2.0 mg L(-1) naphthaleneacetic acid and 0.5 mg L(-1) benzylamino purine. An amino acid mixture of glutamine, arginine, glycine, and aspartic acid promoted sustained protoplast division, with an average plating efficiency of 27%. Protoplast-derived colonies formed callus which readily regenerated shoots on fransfer to Murashige and Skoog based agar medium with 2.0 mg L(-1) indoleacetic acid and 1.0 mg L(-1) benzylamino purine. Leaf explants also showed a marked capacity for shoot regeneration in culture.
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