Related Experiment Video
Updated: May 5, 2026

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
Published on: May 5, 2023
Inhibition of deoxyribonuclease activity associated with soybean chloroplasts
R C Shoemaker1, A G Atherly, R G Palmer
1Department of Genetics, Iowa State University, 50011, Ames, IA, USA.
Abstract:
The effects of spermidine, pH, ethylene diamine tetracetic acid (EDTA), and adenosine triphosphate (ATP) on deoxyribonuclease (DNase) activity associated with the chloroplasts of soybean (Glycine max (L.) Merr.) were investigated. Chloroplast DNase activity was found to be partially inhibited by either 10 mM spermidine, 20 mM EDTA, or 20 mM ATP. DNase activity was also partially inhibited at non-neutral pH's. Nearly complete inhibition was achieved with use of 30 mM EDTA, pH 10, or a combination of 10 mM spermidine and 10 mM EDTA.
Related Concept Videos
Inhibition of Cdk Activity
Inhibition of CDK Activity
Feedback Inhibition
Enzyme Inhibition
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...

