Plant regeneration in vitro from leaf tissues derived from cultured immature embryos of Zea mays L
1International Plant Research Institute, 830 Bransten Road, 94070, San Carlos, CA, USA.
Plant Cell Reports
|November 22, 2013
Abstract:
Maize (Zea mays L.) A188 calluses derived from leaf tissues of in vitro grown seedlings were initiated and maintained on Murashige and Skoog medium supplemented with 2 mg/1 2,4-dichlorophenoxyacetic acid (2,4-D). The calluses produced green leafy structures and subsequently plantlets upon transfer to N6 medium containing 2 mg/1 2,4-D and 0.1 mg/1 zeatin. An embryo-like structure with a green prominent coleoptile and a scutellum-like body was also obtained.
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