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Published on: May 21, 2019
Expression of the plant sulphite reductase in cell suspension cultures from Catharanthus roseus L
1Lehrstuhl für Biochemie der Pflanzen, Abteilung Biologie, ND3/132, Ruhr-Universität, Universitätsstrasse 150, D-4630, Bochum 1, Federal Republic of Germany.
Abstract:
Sulphur-heterotrophic growth exhibited a dual response to the expression of sulphate-assimilating enzymes. The level of ATP-sulphurylase (EC 2.7.7.4) appeared "repressed" while sulphite reductase (EC 1.8.7.1) and O-acetyl-L-serine sulphhydrylase (EC 4.2.99.8) were "derepressed" and coordinated in their occurrence. The capability of the cells to reduce adenylylphosphosulphate or 3'-phospho adenylylphosphosulphate to cysteine coincided with the activity of sulphite reductase. The expression of these reducing steps lacked correlation with the regulation of ATP-sulphurylase.
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