Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glucocorticoids decrease the synthesis of type I procollagen mRNAs.

D Cockayne, K M Sterling, S Shull

    Biochemistry
    |June 3, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function.

    Molecular oral microbiology·2015
    Same author

    The cell envelope proteome of Aggregatibacter actinomycetemcomitans.

    Molecular oral microbiology·2014
    Same author

    Characterization of the secretion pathway of the collagen adhesin EmaA of Aggregatibacter actinomycetemcomitans.

    Molecular oral microbiology·2012
    Same author

    Lipopolysaccharides mediate leukotoxin secretion in Aggregatibacter actinomycetemcomitans.

    Molecular oral microbiology·2012
    Same author

    The extended signal peptide of the trimeric autotransporter EmaA of Aggregatibacter actinomycetemcomitans modulates secretion.

    Journal of bacteriology·2011
    Same author

    Orientation-dependent work function of in situ annealed strontium titanate.

    Journal of physics. Condensed matter : an Institute of Physics journal·2011
    Same journal

    Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

    Biochemistry·2026
    Same journal

    Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

    Biochemistry·2026
    Same journal

    Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

    Biochemistry·2026
    Same journal

    Discovery of Bacterial Unspecific Peroxygenases.

    Biochemistry·2026
    Same journal

    Lactate Biology: Subcellular Routing and Chemical Form Define Function.

    Biochemistry·2026
    Same journal

    Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

    Biochemistry·2026
    See all related articles

    Glucocorticoids, like dexamethasone, reduce procollagen synthesis in chick skin fibroblasts by decreasing procollagen messenger RNA levels. This effect is receptor-mediated and impacts gene expression, not RNA degradation.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Dermatology

    Background:

    • Glucocorticoids are known to influence cellular processes.
    • Procollagen is a key component of the extracellular matrix.
    • Fibroblasts are crucial for skin structure and repair.

    Purpose of the Study:

    • To investigate the effect of glucocorticoids on procollagen synthesis in chick skin fibroblasts.
    • To determine the mechanism by which glucocorticoids inhibit procollagen production.
    • To explore the role of glucocorticoid receptors in this process.

    Main Methods:

    • Treatment of chick skin fibroblasts with dexamethasone.
    • Measurement of procollagen synthesis and beta-actin levels.
    • Analysis of procollagen mRNA levels in different cellular fractions using Northern blot.

    Related Experiment Videos

  • Assessment of receptor mediation using antagonists and agonists.
  • Evaluation of cell growth and RNA degradation rates.

    • Main Results:

    • Dexamethasone selectively decreased procollagen synthesis without affecting beta-actin.
    • The inhibitory effect was linked to reduced type I procollagen messenger RNA levels, particularly in nuclear and cytoplasmic fractions.
    • Glucocorticoid receptor antagonists (progesterone, RU-486) and an agonist (beta-dihydrocortisol) confirmed receptor-mediated effects.
    • Dexamethasone slightly inhibited cell growth but did not alter procollagen mRNA degradation, though total RNA degradation was stabilized.
    • Synthesis of pro alpha 1(I) and pro alpha 2(I) mRNAs was reduced.

    Conclusions:

    • Dexamethasone-induced decrease in procollagen synthesis is mediated by glucocorticoid receptors.
    • The primary mechanism involves the regulation of procollagen gene expression, leading to reduced procollagen mRNA levels.
    • These findings highlight a specific molecular pathway for glucocorticoid action on extracellular matrix production in fibroblasts.