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10:09
A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography HPLC
Published on: March 15, 2017
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Allelochemicals produced during glucosinolate degradation in soil
P D Brown1, M J Morra, J P McCaffrey
1Department of Plant, Soil, and Entomological Sciences, University of Idaho, 83843, Moscow, Idaho.
Journal of Chemical Ecology
|November 22, 2013
Summary
Rapeseed meal in soil releases isothiocyanates and thiocyanate, which quickly suppress plant pests like wireworms. These compounds, derived from glucosinolates, offer a natural pest control method.
Area of Science:
- Agricultural Science
- Soil Science
- Ecology
Background:
- Cruciferous plants suppress soil pests via glucosinolate breakdown products.
- Limited understanding of glucosinolate degradation pathways in soil environments.
Purpose of the Study:
- To investigate glucosinolate degradation in soil.
- To quantify isothiocyanate and thiocyanate production and persistence.
- To correlate chemical changes with wireworm repellency.
Main Methods:
- Soil amended with winter rapeseed meal was analyzed for isothiocyanates and ionic thiocyanate (SCN(-)).
- Concentrations were measured over 100 hours using infrared analysis and ion chromatography.
- Wireworm (Limonius infuscatus) bioassays were conducted concurrently.
Main Results:
- Isothiocyanate levels peaked at 301 nmol/g soil within 2 hours, declining 90% in 24 hours.
- SCN(-) levels peaked at 180 nmol/g soil within 8 hours and persisted longer than isothiocyanates.
- Wireworms were repelled by rapeseed meal within 24 hours, despite the meal not being directly toxic.
Conclusions:
- Rapidly produced isothiocyanates are likely responsible for wireworm repellency.
- Thiocyanate (SCN(-)) may also contribute to pest suppression.
- This study elucidates key mechanisms of crucifer-based soil pest management.
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