Rapid clonal multiplication of Digitalis lanata in tissue culture

Shoot cultures of Digitalis lanata have been established by inoculating the shoot tip of seedlings germinated in aseptic culture, or of field-grown plants, onto Linsmaier and Skoog's RM medium supplemented with 1 mg 6-benzylaminopurine and 0.1 mg indolacetic acid 1(-1). On this medium formation of up to 30 new axillary shoots could be induced. Shoots could be grown into functional plants after root induction on a medium containing reduced amounts (one-fifth of normal) of nitrogen and indolebutyric acid (0.5 mg 1(-1)).