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Bean lectins IV: genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L.
T C Osborn1, J F Manen, J W Brown
1Department of Horticulture, University of Wisconsin, 1575 Linden Drive, 53706, Madison, WI, USA.
Abstract:
Variation in the native conformation of bean lectins was examined using electrophoresis of non-denatured total protein extracts and purified albumin and globulin lectin. The observed variation was related to the genetic variation reported previously for lectin polypeptide composition as revealed by two-dimensional isoelectricfocusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE). When eleven cultivars with different IEF-SDS/PAGE lectin polypeptide compositions were compared, eight had unique non-denatured lectin patterns and three had identical patterns. For some cultivars differences in non-denatured lectin patterns were observed between the purified albumin and globulin lectin preparations.
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